cosmids can accept insert fragments of DNA from 30-50 kilobasesbut plasmids can only accept inserts that are up to 15kb only.
What is the difference between plasmid and bacteriophage lambda?
bacteriophage lambda is a better vector than plasmid as plasmid is small Read More
Somatic embryogenesis is an artificial process that forms an embryo from somatic cells of the plants. The key difference between organogenesis and somatic embryogenesis is that organogenesis is the formation of organs from embryo while somatic embryogenesis is the artificial formation of an embryo from somatic cells.
What is difference between plasmid and chromosomal DNA?
Plasmid is more accurate it shows ur blood type while chromosomal is the dna from your parents. Read More
What is a small double stranded DNA that's used as a vector?
it is a ds DNA use in recombinant DNA technology to insert our interested gene and multiply it.Ex;plasmid,cosmid Read More
What is the similarities and difference between the bacteria and human cells?
a human cells have DNA and bacteria has plasmid Read More
What is the difference between plasmid and transposons?
Plasmids do not usually integrate into the main genomic DNA, but transposons do. Read More
Vectors used in recombinant DNA?
Plasmid,phagemid,virus based vectors,BAC,cosmid and eukaryotic vectors like YAC etc are common vector in r-dna technology.... Read More
What is the difference between plasmid and episomes?
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The… Read More
Difference between a plasmid and a bacterial chromosome?
BACTERIAL CHROMOSOME-genetic material of bacteria involved in replication,transcription and translation process PLASMID-extra chromosomal genetic material Read More
What is the difference between recombination and transformation?
Recombinants take up the plasmid but do not incorporate it into the DNA. Transformants take up the plasmid and integrate it into the DNA. Recombinants wouldn't express the new genes while Transformants will. Read More
What is the difference between a plasmid and a virus?
As far as I know, a plasmid and a virus both consist of small lengths of nucleic acid chains, however a plasmid is confined to a living cell, whereas a virus can have a protective protein coat to protect it in the outside environment and to allow it to be transmitted to other uninfected cells. I think that they both can replicate with cell division. Read More
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes. Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes. Read More
What is the difference between plasmid and vector shall you use all vectors as plasmids?
A vector is a plasmid (usually) that has been engineered to readily accept foreign DNA via recombination. There is also usually special genes previously inserted which code for something that would allow you to distinguish between the colonies which have taken the vector up and which have not, after transformation. A plasmid is the type of DNA baceria usually have - it is circular. There are different names for different sizes and shapes of DNA. Read More
What is the insert capacity of a cosmid vector in DNA cloning?
The insert capacity of a cosmid vector is about 35-45 kb. Read More
What is a plasmid exchange?
Plasmid Exchange is the transfer of genetic material between bacteria through direct cell to cell contact, or through a bridge-like connection between the two cells. Read More
Difference between plasmid DNA isolation and chromosomal DNA isolation?
Ok i think this is right. Plasmid DNA is supercoiled and smaller whereas chromosomal DNA is larger and more fragile. So plasmid DNA isolation is a lot harsher, harsher techniques and harsher chemicals. So maybe some chemicals used in plasmid DNA would break down chromosomal DNA. So basically, the plasmid DNA is tougher and smaller so harsher techniques/chemicals can be used to isolate it. While chromosomal DNA isolation is a 'gentler' way of isolating DNA. Read More
What is the circular DNA that bacteria can share between other bacteria?
What is the difference between genomic DNA and plasmid DNA?
Genome DNA is the primary DNA of a cell and it drives the cell's primary functions. When considering the genome of a lifeform it is only the genomic DNA that is considered. Plasmid DNA is an extra and physically separate chunk of DNA (that usually forms a closed loop) which normally provides some kind of small advantage to the cell. However, the plasmid DNA is not a fundamental part of the host cell and the… Read More
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid. Read More
Example of plasmid?
F-plasmid (fertility plasmid) of any conjugative bateria or Ti-plasmid (tumor-inducing) of Agrobacterium tumaefaciens. Read More
DNA is used to form bonds between the inserted gene and the plasmid DNA?
Genes that encode for drug resistance and can be shared between bacteria are often found on?
What is the function of the Ti plasmid?
What is difference between genomic DNA and chromosomal DNA?
Genomic DNA contains the whole cells DNA, ie Plasmid and chromosomal (in bacteria). mitchondrial and chromosomal. However Chromosomal DNA does not include any of the other DNA types. Read More
What is the difference between the nucleoid and a plasmid?
in microbiology and genetics, a plasmid is a DNA molecule that is separate from, and can replicate independently of, the chromosomal DNA. The nucleoid is an irregularly-shaped region within the cell of a prokaryote that contains all or most of the genetic material Read More
Which is the plasmid that increases resistance to antibiotics?
What plasmid that can degrade toluic acid?
Is expression vector a plasmid?
yes a expresssion vector can be a plasmid Read More
What is meant by curing of plasmid DNA?
Curing a bacteria of plasmid DNA means getting rid of the plasmid so that the cells no longer have it. Read More
The Ti plasmid from which plant pathogen is used to transfer plant genes between species?
What occurs first in the production of a recombinant plasmid?
A plasmid is a type of?
Plasmid is extrachromosomal DNA capable of self replication. Read More
What type of plasmid is the source of the problem with antibiotic resistance?
Do mammalian cell has plasmid DNA?
Plasmid is the property of prokaryotic cell i.e. of bacteria generally no mamalian cell have plasmid but ya there can be chances to have lillte part of DNA sequence to be simillar that of plasmid DNA sequence in prokaryotes Read More
What is a synonym for plasmid?
A plasmid is essentially extra-chromosomal self-replicating DNA Read More
What is a self-transmissible plasmid?
A plasmid which encodes genes for its own transfer. Read More
What is the bacterial cell called when the f factor is in plasmid form?
How many plasmid slots can you have in BioShock 2?
You can have a maximum of 8 plasmid slots. Read More
Puc18 is a vector or a plasmid?
Puc18, i believe, is both. It is a plasmid vector. Plasmid is a more descriptive as it is a type of vector. Similarly to how a cheetah is both a mammal and an animal. Read More
How you clone a gene using cosmid as a vector?
The first step is restriction of the cosmid and the foreign DNA with the restriction enzyme, then ligating the fragments together. Thereafter, the cosmids are loaded into the phage capsid, which leads to the expression of the foreign gene through transduction. Read More
What is a helper plasmid?
A helper plasmid is one that allows for the beginning of replication and transfer of other plasmids from a donor to a recipient. Without a helper plasmid, transposons will not be expressed in the recipient. Read More
Why is ampicillin added to the pound medium?
Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means… Read More
Does an f strain donates a plasmid during conjugation?
Yes, it donates a plasmid to the F negative cell where that plasmid reproduces and makes it own strand in the F negative cell. Read More
How are plasmid classification on their property?
Plasmids are classified as: 1. F plasmid 2. R plasmid 3. Col plasmid F plasmids for fertility factor, it transfers its plasmid to the non fertile making it fertile. R plasmid for certain antibiotic resisitivity..for eg, ampicillin resistance. Col are certain proteins which when produced doesnt let other organisms to invade its cell. Read More
Role of tris edta glucose in plasmid isolation?
role of tris-chloride in plasmid isolation Read More
What do you call a population of cells carrying a desired plasmid?
A plasmid is a small molecule of DNA that replicate independently within the cell. A population of cells carrying a desired plasmid is called a clone. Read More
How is a gene inserted into a plasmid?
Restriction enzymes are used to cut the plasmid open. The gene attaches via 'sticky ends' and the enzyme ligase is used to seal the gene and the plasmid DNA together. Read More
A recombinant plasmid gets inside a bacterial cell by?
A recombinant plasmid gets inside a bacterial cell by Read More
Why does bacteria that contains plasmid glow in uv light?
the plasmid contains a certain gene, which codes for the 'Green Flourescent Protein.' So you put the plasmid in the bacteria, the plasmid starts making that protein in the bacteria, and boom you've got glowing bacteria. works for bunnies and monkeys too, apparently =) Read More
Difference between bacterial cells and human cells?
Bacterial cells are prokaryotic while human cells are eukaryotic. Human cells have a membrane bound nuclues, bacterial cells do not. Human cells have DNA, bacterial cells have plasmid. Cell division occurs differently. Cell shapes differ. Read More
What is recombinant Plasmid?
Recombinant plasmids are engineered plasmid molecules that contain a DNA sequence inserted into it from a foreign source. Read More
Ok i think this is right.
Plasmid DNA is supercoiled and smaller whereas chromosomal DNAis larger and more fragile. So plasmid DNA isolation is a lotharsher, harsher techniques and harsher chemicals. So maybe somechemicals used in plasmid DNA would break down chromosomal DNA.
So basically, the plasmid DNA is tougher and smaller so harshertechniques/chemicals can be used to isolate it. While chromosomalDNA isolation is a 'gentler' way of isolating DNA.
What is difference between plasmid and chromosomal DNA?
Plasmid is more accurate it shows ur blood type while chromosomal is the dna from your parents. Read More
What is the difference between plasmid and episomes?
Plasmid DNA is an extra-chromosomal DNA molecule, it cannot link up with chromosomal DNA, and it contains the genetic informations that are necessary for its own replication. Episomes is any kind of extra-chromosomal DNA that can link up with chromosomal DNA. That is the main difference between them two. Episomes are usually larger than other extra-chromosomal DNA. An example of episome are the viruses, because they intergrate their genetic material into the host's chromosomal DNA.The… Read More
What is difference between genomic DNA and chromosomal DNA?
Genomic DNA contains the whole cells DNA, ie Plasmid and chromosomal (in bacteria). mitchondrial and chromosomal. However Chromosomal DNA does not include any of the other DNA types. Read More
Difference between a plasmid and a bacterial chromosome?
BACTERIAL CHROMOSOME-genetic material of bacteria involved in replication,transcription and translation process PLASMID-extra chromosomal genetic material Read More
What are plasmid genes?
A plasmid is an extra chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently from the chromosomal DNA http://en.wikipedia.org/wiki/Plasmid I think this is far use. Read More
Role of tris edta glucose in plasmid isolation?
role of tris-chloride in plasmid isolation Read More
What is a synonym for plasmid?
A plasmid is essentially extra-chromosomal self-replicating DNA Read More
What occurs first in the production of a recombinant plasmid?
What is the difference between plasmid and bacteriophage lambda?
bacteriophage lambda is a better vector than plasmid as plasmid is small Read More
What is the difference between the original plasmid and the recombinant plasmid?
Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes. Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes. Read More
How plasmid DNA and genomic DNA are separated during plasmid isolation?
a restriction enzyme help to cut the DNA Read More
Function of glucose in plasmid isolation?
Why do you use Tris Hcl in plasmid isolation?
What is the difference between the nucleoid and a plasmid?
in microbiology and genetics, a plasmid is a DNA molecule that is separate from, and can replicate independently of, the chromosomal DNA. The nucleoid is an irregularly-shaped region within the cell of a prokaryote that contains all or most of the genetic material Read More
Use of 70 percent ethanol in plasmid isolation?
Ethanol is a dehydrating agent that is able to precipitate DNA during the isolation process Read More
Function of TE buffer in plasmid isolation?
It protects DNA or RNA from degradation. Read More
Role of Glacial acetic acid in plasmid isolation?
Why you use LiCl in plasmid isolation by telt method?
Function of NaOH in isolation plasmid?
Plasmid isolation involves growing the plasmid under conditions that are suitable for genes to come into play. For example the gene for ampicillin resistance; the bacteria with plasmids are placed with ampicillin so their genes can be seen for those who survived. Sodium hydroxide acts a detergent in the extraction process. A detergent's main role is to break down cell walls and cell membranes. How so? They act as poking holes into membranes. However, for… Read More
What is the difference between plasmid and transposons?
Plasmids do not usually integrate into the main genomic DNA, but transposons do. Read More
What is the similarities and difference between the bacteria and human cells?
a human cells have DNA and bacteria has plasmid Read More
Function of phenol chloroform in plasmid isolation?
removes the remaining protein which is left after denaturation Read More
How is plasmid DNA separated from chromosomal DNA?
Plasmid DNA can be separated from chromosomal DNA by the alkaline lysis method. Cells are first lysed with a strong alkaline buffer containing SDS and NaOH. A series of steps involving precipitation, centrifugation and running the supernatant through a resin filter seperates cellular debris as well as chomosomal DNA form the plasmid. This is most often done very easily using mini, midi or maxi-prep kits which are available from companies. From there the plasmid DNA… Read More
Circular extra genomic DNA is called?
A circular band of DNA that exists separate of chromosomal DNA is called a plasmid. Plasmids reside within bacteria cells and can replicate independently of the chromosomal DNA. Read More
What is the most important tools used by biologists?
plasmid - is a DNA molecule that is separate from,and can replicate independently of,the chromosomal DNA. Read More
Function of EDTA in plasmid isolation?
It inhibits nucleases leading to the rupture of cell wall and membrane. Read More
Role of NaOH in plasmid DNA isolation?
To separate the bacterial chromosomal DNA and sheared DNA from plasmid DNA, NaOH is often used. Chromosomal DNA and sheared DNA are both linear, whereas plasmid DNA is circular. When the solution is basic, for example, when sodium hydroxide is added, double-stranded DNA molecules separate. This is known as denaturation. Their complementary bases are no longer associated with each other. This can be thought of much like the two complementary sides of a zipper. When… Read More
Function of alcohol in plasmid DNA isolation?
Alcohol is a dehydrating agent that can disrupt proteins in the cell membrane. This breaks open the cell and liberates the plasmid(s) into the bulk of the solutuion Read More
What do plasmids look like?
Plasmid are double helix cirular ring shaped extra chromosomal DNA present in some of microbes. Read More
What is the Role of RNAase in plasmid preparation?
In plasmid isolation RNA behaves as an unwanted material so to separate it out RNAase is required which breaks down the RNA. This is done to get pure quality of the product. Read More
What is the function of phenol chloroform isoamyl alcohol in plasmid isolation?
it helps in the removal of proteins from nucleic acid Read More
What is the difference between recombination and transformation?
Recombinants take up the plasmid but do not incorporate it into the DNA. Transformants take up the plasmid and integrate it into the DNA. Recombinants wouldn't express the new genes while Transformants will. Read More
What is alkaline lysis method of plasmid isolation?
is isolation of plasmids by some alkaline substances like SDS and NaoH with some buffer and some substances that maintenance on the osmotic pressure. Read More
Do all microbes have plasmids?
No,all the microbes do not have a plasmid. Plasmids are present only in a bacterial cell.Plasmids are the extra chromosomal DNA which can replicate by itself. Read More
If a piece of DNA breaks off from a chromosome and attaches itself to a non homologous chromosome at another location what type of change has occurred?
The change that has occurred is chromosomal translocation, probably involving plasmid-type vectors. When a similar process occurs under complete chromosomal control, it is called chromosomal recombination. Read More
What is the use of alkaline lysis solution 1 in plasmid isolation?
it helps to homogenize the cell and give single cell suspension Read More
Role of tris HCl in plasmid isolation?
The role that tris-HCI plays in plasmid isolation is to maintain the pH of the solution. This prevents degradation of the plasmids. Tris stands for the organic compound, tris(hydroxymethyl)aminomethane, which is a common pH buffer. HCl is a salt acid called hydrochloride. This is added as a buffer as well to add stabilization. Read More
What type of DNA do restriction enzymes cut?
Restriction enzymes can cut any DNA (plasmid or chromosomal), as long as the enzymes work in suitable acidity and temperatures. Read More
Why do bacteria have extra chromosomal DNA?
Extra chromosomal DNA - plasmids- are useful to bacteria as they possess favourable genes conferring resistance to certain toxins/antibiotics and/or assist with the survival of the bacteria through metabolic benefits. The extra chromosomal DNA is only maintained if it has a purpose so a plasmid conferring antibiotic resistance will not be maintained by a bacterium in an antibiotic free environment... Read More
Function of SDS in plasmid isolation?
SDS is an ionic detergent which disrupts the phospholipids and protein components of the cell membrane, releasing the cellular components. Read More
What is the use of phenol during plasmid isolation?
Phenol is an organic solvent that creates a hydrophobic environment for proteins to precipitate. The precipitated proteins can later be removed through centrifugation thus allowing the easy isolation of DNA from the cell Read More
What is the difference between a plasmid and a virus?
As far as I know, a plasmid and a virus both consist of small lengths of nucleic acid chains, however a plasmid is confined to a living cell, whereas a virus can have a protective protein coat to protect it in the outside environment and to allow it to be transmitted to other uninfected cells. I think that they both can replicate with cell division. Read More
Does bacteria have plasmids?
Bacteria carry plasmids which is a double stranded DNA . It is how their extra chromosomal DNA is stored, they also have chromosomal DNA. Plasmids are extra-chromosomal DNA in Bacterial cells that replicate independently in cell. Plasmids are ubiquitous- means significant number of bacteria have plasmid and can have more that one plasmids. But Plasmids do not occur in all bacterial isolates. Read More
Function of isopropanol and ethanol in plasmid isolation?
Cold ethanol or isopropanol is used to precipitate the plasmid DNA, DNA is insoluble in alcohol and clumps or clings together. Centrifuging will cause the precipitate to form a pellet which can be decanted from the unwanted supernatant. Where as if compared with RNA isolation isopropanol is less efficient in precipitating RNA, where in presence of Lithium chloride or ammonium ions can give a good yield Read More
Where is plasmid located within your body?
A circular, double-stranded unit of DNA that replicates within a cell independently of the chromosomal DNA. Plasmids are most often found in bacteria and are used in recombinant DNA research to transfer genes between cells. Read More
What is curing of plasmid?
Bacteria possess extra chromosomal DNA,called plasmids. Often it carries functional genes for the resistance of bacteria (example: Aromotic compound degrading genes). Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin or acridine orange! Read More
What is the difference between plasmid and vector shall you use all vectors as plasmids?
A vector is a plasmid (usually) that has been engineered to readily accept foreign DNA via recombination. There is also usually special genes previously inserted which code for something that would allow you to distinguish between the colonies which have taken the vector up and which have not, after transformation. A plasmid is the type of DNA baceria usually have - it is circular. There are different names for different sizes and shapes of DNA. Read More
What is the difference between cosmid and plasmid?
cosmids can accept insert fragments of DNA from 30-50 kilobases but plasmids can only accept inserts that are up to 15kb only. Read More
The function of Wash solution in plasmid DNA extraction?
Plasmid isolation has a step called washing step that carried out in the column in which the plasmid DNA are already bind. There are two wash solution, first one endo wash buffer that wash the traces of bacterial membrane remnants such as LPS. Wash buffer two has ethanol wash off any protein contaminants present on the column. These wash steps ensure the purify of isolated plasmid DNA. Read More
What is a plasmid exchange?
Plasmid Exchange is the transfer of genetic material between bacteria through direct cell to cell contact, or through a bridge-like connection between the two cells. Read More